Zebrafish vs Denticle herring LastZ results
Zebrafish (Danio rerio, GRCz11) and Denticle herring (Denticeps clupeoides, fDenClu1.1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 98. Zebrafish was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Zebrafish | Denticle herring |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 364,655 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Zebrafish |
Uncovered: 1,300,318,363 out of 1,373,471,384 |
Uncovered: 7,622,031 out of 42,031,845 |
| Denticle herring |
Uncovered: 488,821,415 out of 567,401,054 |
Uncovered: 7,237,105 out of 41,411,314 |
Block size distribution
| Size range | All 364,655 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 2,346 |
10.7 kb |
|||
| 10 bp - 100 bp | 55,969 |
4.0 Mb |
17,111 |
1.3 Mb |
|
| 100 bp - 1 kb | 299,316 |
78.4 Mb |
75,407 |
23.4 Mb |
|
| 1 kb - 10 kb | 7,008 |
11.3 Mb |
13,986 |
36.8 Mb |
|
| 10 kb - 100 kb | 14 |
224.8 kb |
1,190 |
26.5 Mb |
|
| 100 kb - 1 Mb | 2 |
245.4 kb |
38 |
6.2 Mb |
|