American bison (Bison_UMD1.0)

American bison assembly and gene annotation

Assembly

The Bison_UMD1.0 assembly was submitted by University of Maryland on October 2014. The assembly is on scaffold level, consisting of 470,415 contigs assembled into 128,431 scaffolds. The N50 size is the length such that 50% of the assembled genome lies in blocks of the N50 size or longer. The N50 length for the contigs is 19,971 while the scaffold N50 is 7,192,658.

Gene annotation

The gene annotation process was carried out using a combination of protein-to-genome alignments, annotation mapping from a suitable reference species and RNA-seq alignments (where RNA-seq data with appropriate meta data were publicly available). For each candidate gene region, a selection process was applied to choose the most appropriate set of transcripts based on evolutionary distance, experimental evidence for the source data and quality of the alignments.
Small ncRNAs were obtained using a combination of BLAST and Infernal/RNAfold.
Pseudogenes were calculated by looking at genes with a large percentage of non-biological introns (introns of <10bp), where the gene was covered in repeats, or where the gene was single exon and evidence of a functional multi-exon paralog was found elsewhere in the genome.
lincRNAs were generated via RNA-seq data where no evidence of protein homology or protein domains could be found in the transcript.

In accordance with the Fort Lauderdale Agreement, please check the publication status of the genome/assembly before publishing any genome-wide analyses using these data.

More information

General information about this species can be found in Wikipedia.

Statistics

Summary

AssemblyBison_UMD1.0, INSDC Assembly GCA_000754665.1, Oct 2014
Base Pairs2,953,622,319
Golden Path Length2,953,622,319
Annotation providerEnsembl
Annotation methodFull genebuild
Genebuild startedOct 2018
Genebuild releasedApr 2019
Genebuild last updated/patchedMar 2020
Database version111.1

Gene counts

Coding genes20,241
Non coding genes4,598
Small non coding genes4,188
Long non coding genes8
Misc non coding genes402
Pseudogenes670
Gene transcripts34,001

Other

Genscan gene predictions61,520