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Ocean sunfish assembly and gene annotation

Assembly

The ASM169857v1 assembly was submitted by BGI-shenzhen on August 2016. The assembly is on scaffold level, consisting of 51,826 contigs assembled into 5,552 scaffolds. The N50 size is the length such that 50% of the assembled genome lies in blocks of the N50 size or longer. The N50 length for the contigs is 23,239 while the scaffold N50 is 8,766,736.

The genome assembly represented here corresponds to GenBank Assembly ID GCA_001698575.1

Gene annotation

The ocean sunfish or common mola (Mola mola) is the heaviest known bony fish in the world. Adults typically weigh between 247 and 1,000 kg (545–2,205 lb). The species is native to tropical and temperate waters around the globe. It resembles a fish head with a tail, and its main body is flattened laterally. Sunfish can be as tall as they are long when their dorsal and ventral fins are extended.Sunfish live on a diet consisting mainly of sea jellies, but because this diet is nutritionally poor, they consume large amounts to develop and maintain their great bulk. Females of the species can produce more eggs than any other known vertebrate, up to 300,000,000 at a time. Sunfish fry resemble miniature pufferfish, with large pectoral fins, a tail fin, and body spines uncharacteristic of adult sunfish.Adult sunfish are vulnerable to few natural predators, but sea lions, killer whales, and sharks will consume them. Among humans, sunfish are considered a delicacy in some parts of the world, including Japan, Korea, and Taiwan. In the EU, regulations ban the sale of fish and fishery products derived from the family Molidae. Sunfish are frequently caught in gillnets.A member of the order Tetraodontiformes, which also includes pufferfish, porcupinefish, and filefish, the sunfish shares many traits common to members of this order. The ocean sunfish, Mola mola, is the type species of the genus.

The gene annotation process was carried out using a combination of protein-to-genome alignments, annotation mapping from a suitable reference species and RNA-seq alignments (where RNA-seq data with appropriate meta data were publicly available). For each candidate gene region, a selection process was applied to choose the most appropriate set of transcripts based on evolutionary distance, experimental evidence for the source data and quality of the alignments.
Small ncRNAs were obtained using a combination of BLAST and Infernal/RNAfold.
Pseudogenes were calculated by looking at genes with a large percentage of non-biological introns (introns of <10bp), where the gene was covered in repeats, or where the gene was single exon and evidence of a functional multi-exon paralog was found elsewhere in the genome.
lincRNAs were generated via RNA-seq data where no evidence of protein homology or protein domains could be found in the transcript.

In accordance with the Fort Lauderdale Agreement , please check the publication status of the genome/assembly before publishing any genome-wide analyses using these data.

More information

General information about this species can be found in Wikipedia.

Statistics

Summary

AssemblyASM169857v1, INSDC Assembly GCA_001698575.1, Aug 2016
Base Pairs639,451,992
Golden Path Length639,451,992
Annotation providerEnsembl
Annotation methodFull genebuild
Genebuild startedMay 2018
Genebuild releasedJul 2018
Genebuild last updated/patchedJul 2018
Database version94.1

Gene counts

Coding genes21,404
Non coding genes444
Small non coding genes425
Long non coding genes2
Misc non coding genes17
Pseudogenes45
Gene transcripts29,504

Other

Genscan gene predictions34,553

About this species