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Midas cichlid assembly and gene annotation

Assembly

The Midas_v5 assembly was submitted by University Of Konstanz on September 2014. The assembly is on scaffold level, consisting of 67,543 contigs assembled into 6,637 scaffolds. The N50 size is the length such that 50% of the assembled genome lies in blocks of the N50 size or longer. The N50 length for the contigs is 23,456 while the scaffold N50 is 1,216,136.

The genome assembly represented here corresponds to GenBank Assembly ID GCA_000751415.1

Gene annotation

Amphilophus citrinellus is a large cichlid fish endemic to the San Juan River and adjacent watersheds in Costa Rica and Nicaragua. In the aquarium trade A. citrinellus is often sold under the trade name of Midas cichlid. A. citrinellus are omnivorous and their diet consists of plant material, molluscs and smaller fish. The species is closely related to but not to be mistaken as Amphilophus labiatus.

The gene annotation process was carried out using a combination of protein-to-genome alignments, annotation mapping from a suitable reference species and RNA-seq alignments (where RNA-seq data with appropriate meta data were publicly available). For each candidate gene region, a selection process was applied to choose the most appropriate set of transcripts based on evolutionary distance, experimental evidence for the source data and quality of the alignments.
Small ncRNAs were obtained using a combination of BLAST and Infernal/RNAfold.
Pseudogenes were calculated by looking at genes with a large percentage of non-biological introns (introns of <10bp), where the gene was covered in repeats, or where the gene was single exon and evidence of a functional multi-exon paralog was found elsewhere in the genome.
lincRNAs were generated via RNA-seq data where no evidence of protein homology or protein domains could be found in the transcript.

In accordance with the Fort Lauderdale Agreement , please check the publication status of the genome/assembly before publishing any genome-wide analyses using these data.

More information

General information about this species can be found in Wikipedia.

Statistics

Summary

AssemblyMidas_v5, INSDC Assembly GCA_000751415.1, Sep 2014
Base Pairs844,902,565
Golden Path Length844,902,565
Annotation providerEnsembl
Annotation methodFull genebuild
Genebuild startedMay 2018
Genebuild releasedJul 2018
Genebuild last updated/patchedJul 2018
Database version94.5

Gene counts

Coding genes23,696
Non coding genes782
Small non coding genes760
Long non coding genes3
Misc non coding genes19
Pseudogenes50
Gene transcripts32,597

Other

Genscan gene predictions36,489

About this species